Sains Malaysiana 53(1)(2024): 171-188

http://doi.org/10.17576/jsm-2024-5301-14

 

Kesan Transglutaminase kepada Ciri Fizikokimia dan Aktiviti Biologi Hidrolisat Sarang Burung Walit

(Effect of Transglutaminase towards Physicochemical Properties and Biological Activities of Edible Bird’s Nest Hydrolysates)

 

KOH YUN SHI1, TER ZHI YIN1, RAFIDAH MOHD ARIFF1,4, NUR FARHANA ABD RAHMAN1,5, CHANG LEE SIN2,3, ABDUL SALAM BABJI1,2 & LIM SENG JOE1,2,*

 

1Jabatan Sains Makanan, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

2Pusat Inovasi Teknologi Manisan (MANIS), Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

3Jabatan Sains Makanan dan Nutrisi, Fakulti Sains Gunaan, Universiti UCSI, No.1, Jalan Menara Gading, UCSI Heights, 56000 Cheras, Kuala Lumpur, Malaysia

 4International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM), 53100 Jalan Gombak, Kuala Lumpur, Malaysia

5 School of Industrial Technology, Faculty of Applied Sciences, UiTM Shah Alam, Shah Alam 40450, Malaysia

 

Received: 9 March 2023/Accepted: 8 December 2023

 

Abstrak

Kajian terdahulu menunjukkan bahawa glikoprotein sarang burung walit boleh dihidrolisis dan dipulihkan sebagai hidrolisat sarang burung walit (SBWh) yang boleh dimakan dalam bentuk serbuk. Kajian ini adalah kerja sambungan daripada kajian terdahulu, yang mana pelbagai kepekatan enzim transglutaminase (TG) telah digunakan dalam pembentukan semula bentuk serbuk SBWh menggunakan acuan dan kesannya terhadap sifat fizikokimia dan bioaktiviti SBWh. Proses hidrolisis enzim menunjukkan bahawa sampel SBWh mempunyai hasil pulih lebih daripada 60% dan menunjukkan warna yang lebih terang daripada SBW mentah. Kelarutan SBWh dengan TG lebih rendah tetapi ia boleh dibentuk semula ke bentuk yang diingini. Corak spektrum FTIR untuk sampel SBWh juga menunjukkan bahawa ketulenan glikopeptida SBW yang tinggi telah diperoleh. Untuk aktiviti antioksidan, produk sampingan SBWh (SBWhcp) menunjukkan aktiviti penyingkiran radikal bebas DPPH yang lebih tinggi. Secara ringkasnya, penambahan TG kepada SBWh telah meningkatkan sifat fizikokimia dan bioaktiviti. Penemuan ini menghasilkan wawasan tentang aplikasi dan pembangunan produk EBN menggunakan EBNh dengan TG yang mempunyai bioaktiviti yang tinggi.

 

Kata kunci: DPPH; gam daging; hidrolisat; sarang burung walit

 

Abstract

Previous study has shown that edible bird’s nest (EBN) glycoprotein can be hydrolysed and recovered as edible bird’s nest hydrolysate (EBNh) in the form of powder. This research is a continuous work from previous study, in which different concentrations of transglutaminase enzyme (TG) was applied in reconstituting the shape of EBNh powder using mould, and its effect on the physicochemical properties and bioactivities of EBNh. Enzymatic hydrolysis process showed that EBNh samples had recovery yield exceeding 60% and demonstrated brighter colour than raw EBN. Solubility of EBNh with TG was lower but it can be reconstituted into desirable shape. Spectrum pattern of FTIR for EBNh sample also showed that high authenticity of EBN glycopeptide was obtained. For antioxidant activities, EBNh co-product (EBNhcp) exhibited higher DPPH free radical scavenging activity. In short, addition of TG to EBNh has enhanced the physicochemical properties and bioactivities. The finding generates insight on the application and development of EBN products using EBNh with TG with high bioactivities.

 

Keywords: DPPH; edible bird’s nest; hydrolysate; meat glue

 

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*Corresponding author; email: joe@ukm.edu.my

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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